Role of NBCe1 and AE2 in secretory ameloblasts.
J Dent Res
; 87(4): 391-5, 2008 Apr.
Article
en En
| MEDLINE
| ID: mdl-18362326
ABSTRACT
The H(+)/base transport processes that control the pH of the microenvironment adjacent to ameloblasts are not currently well-understood. Mice null for the AE2 anion exchanger have abnormal enamel. In addition, persons with mutations in the electrogenic sodium bicarbonate co-transporter NBCe1 and mice lacking NBCe1 have enamel abnormalities. These observations suggest that AE2 and NBCe1 play important roles in amelogenesis. In the present study, we aimed to understand the roles of AE2 and NBCe1 in ameloblasts. Analysis of the data showed that NBCe1 is expressed at the basolateral membrane of secretory ameloblasts, whereas AE2 is expressed at the apical membrane. Transcripts for AE2a and NBCe1-B were detected in RNA isolated from cultured ameloblast-like LS8 cells. Our data are the first evidence that AE2 and NBCe1 are expressed in ameloblasts in vivo in a polarized fashion, thereby providing a mechanism for ameloblast transcellular bicarbonate secretion in the process of enamel formation and maturation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Antiportadores
/
Proteínas de Transporte de Anión
/
Simportadores de Sodio-Bicarbonato
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Ameloblastos
/
Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
En
Revista:
J Dent Res
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos