[Cell adhesion in neoplastic cells and syngeneic fibroblasts of the rat: effect of progesterone and estradiol]. / Adesione cellulare in cellule neoplastiche ed in fibroblasti singeneici di ratto: effetto del progesterone e dell'estradiolo.

ABSTRACT

Recent advances in the study on the importance of steroid receptors in the treatment of hormone-responsive tumors established the relationship between plasma membrane and the first steps of the cellular response to the endocrine stimulus. We have therefore found interesting to study the influence of estrogen and progesterone on cell adhesion of neoplastic (SGS/3A) and normal syngeneic (FG) cells. We demonstrated the presence of a nuclear estrogenic and a cytosolic progestin receptor in the SGS/3A cells. In syngeneic fibroblasts both receptors are absent. Cell adhesion kinetics obtained determining the percent of single labeled cells with 3H-leucine adhering to confluent monolayer (cell-cell adhesion) showed that the physiological concentrations of estradiol and progesterone induce an inhibition of cell-substratum adhesion (25-30%) in neoplastic cells, but do not influence cell-cell adhesion. In FG cells the two hormones cause an increase of cell-substratum adhesion (35-40%), but do not influence cell-cell adhesion. Results suggest that FG cells, although lacking cytoplasmic or nuclear receptors for estradiol and progesterone, probably have other steroid-receptive molecules involved in adhesive processes on their cell-surface.