Memy I: a novel murine model for uterine leiomyoma using adenovirus-enhanced human fibroid explants in severe combined immune deficiency mice.
Am J Obstet Gynecol
; 199(2): 156.e1-8, 2008 Aug.
Article
en En
| MEDLINE
| ID: mdl-18468574
ABSTRACT
OBJECTIVE:
This study was undertaken to develop a representative murine model for human leiomyoma. STUDYDESIGN:
Human fibroid tumor tissues were cut into small pieces and treated with medium alone, adenoviral-beta-galactosidase, adenoviral-vascular endothelial growth factor-A, adenoviral-cyclooxygenase-2, or both adenoviral-vascular endothelial growth factor-A and adenoviral- cyclooxygenase-2. Tissue pieces were inserted subcutaneously in the flank of each severe combined immunodeficient mouse. The developed lesion was measured twice per week. Xenograft tissues were harvested after 30 days and analyzed.RESULTS:
Tissue pieces transfected with both adenoviral-cyclooxygenase-2 and adenoviral-vascular endothelial growth factor-A continued to grow up to 30 days postimplantation. The number of proliferating and apoptotic cells, as well as the expression of smooth muscle actin, desmin, vimentin, estrogen receptors, and progesterone receptors was similar between retrieved grafts from that group and the original patient tissue. Furthermore, hematoxylin and eosin and Masson's Trichrome stains confirmed this similarity.CONCLUSION:
Human uterine leiomyoma xenografts, pretreated with both adenoviral- cyclooxygenase-2 and adenoviral-vascular endothelial growth factor-A and implanted subcutaneously in severe combined immunodeficient mice, represent a novel model for human uterine leiomyoma.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Uterinas
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Modelos Animales de Enfermedad
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Leiomioma
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Animals
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Female
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Humans
Idioma:
En
Revista:
Am J Obstet Gynecol
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos