Negatively charged phospholipids restore prePhoE translocation across phosphatidylglycerol-depleted Escherichia coli inner membranes.
J Biol Chem
; 266(14): 8659-62, 1991 May 15.
Article
en En
| MEDLINE
| ID: mdl-1851153
ABSTRACT
Translocation of outer membrane precursor proteins across the Escherichia coli inner membrane is severely hampered in lipid biosynthetic mutants with strongly reduced phosphatidylglycerol (PG) levels (De Vrije, T., De Swart, R. L., Dowhan, W., Tommassen, J., and De Kruijff, B. (1988) Nature 334, 173-175; Lill, R., Dowhan, W., and Wickner, W. (1990) Cell 60, 271-280). Two independent methods were used to demonstrate that anionic lipids by virtue of their negative head-group charge are involved in membrane translocation of the precursor of the pore protein PhoE. Using a lipid transfer protein-based method we show that introduction from lipid vesicles of PG and other acidic phospholipids but not of phosphatidylcholine restores efficient translocation across the membrane of PG-depleted inner membrane vesicles. Moreover, translocation was found to be proportional to the PG content in vesicles isolated from strain HDL11 in which the PG content was altered by varying the synthesis of the PG-phosphate synthase.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfatidilgliceroles
/
Fosfolípidos
/
Proteínas de la Membrana Bacteriana Externa
/
Membrana Celular
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Escherichia coli
Idioma:
En
Revista:
J Biol Chem
Año:
1991
Tipo del documento:
Article