Anthracycline immunoconjugates prepared by a site-specific linkage via an amino-dextran intermediate carrier.
Cancer Res
; 51(16): 4192-8, 1991 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-1868440
ABSTRACT
Anthracycline, either daunomycin or doxorubicin, was site specifically attached to the carbohydrate moiety of a monoclonal anticarcinoembryonic antigen antibody by using amino-dextran as the intermediate carrier. The reaction resulted in an immunoconjugate that contains approximately 20 to 25 molecules of drug per molecule of immunoglobulin G. Flow-cytometric studies revealed the retention of the antibody-binding activity. The immunoconjugate was cytotoxic to the target cells, as examined by the 75selenomethionine incorporation studies, and remained efficient for targeting a human colonic tumor (GW-39) in the nude mouse model. The conjugate possessed a greater antitumor activity against the subcutaneous tumor than either the free drug or an irrelevant antibody conjugate, and it was well tolerated by the animals at a much higher dose level than was the unconjugated drug.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Daunorrubicina
/
Doxorrubicina
/
Inmunotoxinas
/
Neoplasias del Colon
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1991
Tipo del documento:
Article