Bacterial DNA sample preparation from whole blood using surface-modified Si pillar arrays.
Anal Chem
; 80(20): 7786-91, 2008 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-18729477
ABSTRACT
A novel bacterial DNA sample preparation device for molecular diagnostics has been developed. On the basis of optimized conditions for bacterial adhesion, surface-modified silicon pillar arrays for bacterial cell capture were fabricated, and their ability to capture bacterial cells was demonstrated. The capture efficiency for bacterial cells such as Escherichia coli, Staphylococcus epidermidis, and Streptococcus mutans in buffer solution was over 75% with a flow rate of 400 microL/min. Moreover, the proposed method captured E. coli cells present in 50% whole blood effectively. The captured cells from whole blood were then in- situ lyzed on the surface of the microchip, and the eluted DNA was successfully amplified by qPCR. These results demonstrate that the full process of pathogen capture to DNA isolation from whole blood could be automated in a single microchip.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Silicio
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ADN Bacteriano
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Métodos Analíticos de la Preparación de la Muestra
Idioma:
En
Revista:
Anal Chem
Año:
2008
Tipo del documento:
Article