Iron(III) uptake and release by chrysobactin, a siderophore of the phytophatogenic bacterium Erwinia chrysanthemi.
Inorg Chem
; 47(20): 9419-30, 2008 Oct 20.
Article
en En
| MEDLINE
| ID: mdl-18803373
ABSTRACT
The plant pathogenic enterobacterium Erwinia chrysanthemi causes important soft-rot disease on a wide range of plants including vegetables and ornamentals of economic importance. It produces a major mono(catecholate) siderophore, chrysobactin (alpha-N-(2,3-dihydroxybenzoyl)-D-lysyl-L-serine). To unravel the role of chrysobactin in the virulence of E. chrysanthemi, its iron(III) coordination properties were thus investigated in aqueous solutions using electrospray ionization mass spectrometric, potentiometric, and spectrophotometric methods. Moreover, kinetic experiments allowed us to determine the uptake and release mechanisms. The formation mechanism of the 11 complex reveals a key role of the terminal carboxylic group of chrysobactin in the binding of either FeOH(2+) or Fe2(OH)2(4+). The proton-driven dissociation of the ferric tris-, bis-, and mono(chrysobactin) complexes was also studied. For these three ferric complexes, a single protonation triggers the release of the bound chrysobactin molecule. Interestingly, the dissociation of the last ligand proceeded via the formation of an intermediate for which a salicylate-type mode of bonding was proposed.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Dickeya chrysanthemi
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Sideróforos
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Dipéptidos
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Hierro
Idioma:
En
Revista:
Inorg Chem
Año:
2008
Tipo del documento:
Article
País de afiliación:
Francia