Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome.
Mol Cell Biol
; 29(1): 150-6, 2009 Jan.
Article
en En
| MEDLINE
| ID: mdl-18936163
ABSTRACT
We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains approximately 1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1-immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cromosoma X
/
Histonas
/
Inactivación del Cromosoma X
Límite:
Animals
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Female
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Humans
/
Male
Idioma:
En
Revista:
Mol Cell Biol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia