[Effect of silver-hydroxyapatite/titania nanocomposites on proliferation, vitality and ultrastructure of osteoblasts].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 39(5): 766-8, 775, 2008 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-19024309
ABSTRACT
OBJECTIVE:
Osteoblasts behaviors were investigated when cultured in the presence of silver-hydroxyapatite/titania nanoparticles (nAg-HA/TiO2) in vitro.METHODS:
Rat calvaria osteoblasts were cultured in the presence of 10 mirog/mL-500 mirog/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope (TEM).RESULTS:
Both cell proliferation and vitality were restricted by addition of nanoparticles, while the respiration rates as an index for unit cell viability were enhanced. nAg-HA/TiO2 at low concentration showed superiority. No remarkable ultrastructural changes were observed in the osteoblasts exposed to nanoparticles. The differences in cell proliferation and cell vitality between nAg-HA/TiO2 group and nHA group at the same concentration were insignificant.CONCLUSION:
nAg-HA/TiO2 restricted cells proliferation, but triggered unit cell viability. Cytocompatibility of nAg-HA/TiO2 is almost the same as that of nHA's.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Plata
/
Titanio
/
Durapatita
/
Nanocompuestos
Límite:
Animals
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2008
Tipo del documento:
Article
País de afiliación:
China