[BFGF entraped in cationic liposomes as adjuvant: optimization of technical parameters].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 39(5): 839-41, 846, 2008 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-19024328
ABSTRACT
OBJECTIVE:
To test the immune efficiency of bFGF entraped in cationic liposomes as adjuvant in vivo.METHODS:
The technical parameters on encapsulation were tested in each step to gain high encapsulation efficiencies, which included lipid composition, weight ratio of protein and lipids, liposome extrusion, and different conditions of freeze-thawing. The bFGF in cationic liposome, Freund's adjuvant, or PBS were injected (four times) to the four-week old Balb/c mice to test the immune responses. The serum antibody was measured by ELISA 13 days after each injection.RESULTS:
Maximal encapsulation efficiency (about 50%) was achieved through optimized technical parameters. Cationic liposome demonstrated satisfied immune efficiency as adjuvant.CONCLUSION:
Cationic liposome is a safe and effective immunological adjuvant.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Factor 2 de Crecimiento de Fibroblastos
/
Inmunización
/
Liposomas
Límite:
Animals
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2008
Tipo del documento:
Article
País de afiliación:
China