Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography.
Biochim Biophys Acta
; 483(2): 487-91, 1977 Aug 11.
Article
en En
| MEDLINE
| ID: mdl-19074
ABSTRACT
A novel procedure for the purification of methylmalonyl-CA mutase from Propionibacterium shermanii has been described which employs affinity chromatography on a column of immobilized vitamin B-12 linked covalently to Sepharose. The method has the advantage of being simple and rapid, thus enabling the purification of the enzyme to near homogeneity with good yields.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Propionibacterium
/
Isomerasas
/
Metilmalonil-CoA Mutasa
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1977
Tipo del documento:
Article