Measurement of DNA morphological parameters at highly entangled regime on surfaces.
J Phys Chem B
; 113(15): 4987-90, 2009 Apr 16.
Article
en En
| MEDLINE
| ID: mdl-19309127
ABSTRACT
The morphology of circular DNA deposited from a solution on the mica surface is analyzed from the power spectrum density (PSD) of the atomic force microscopy (AFM) images. Sample morphology is modulated in a broad range of concentration C from isolated molecules to highly entangled networks. DNA exhibits a multiaffine behavior with two correlation length scales the persistence length P which remains constant ( approximately 50 nm) within the C range and the intermolecular distance xi which exhibits a decay with increasing C. Applying a diffusion based model in which xi scales as xi approximately D(-0.25).C(-0.5), we extracted the DNA diffusion coefficient D approximately 2 x 10(-7) cm(2)/s. This value is consistent with a high-molecular-weight plasmid DNA supercoiled in the solution.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN Circular
/
Silicatos de Aluminio
/
Conformación de Ácido Nucleico
Idioma:
En
Revista:
J Phys Chem B
Asunto de la revista:
QUIMICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Italia