Gold, poly(beta-amino ester) nanoparticles for small interfering RNA delivery.
Nano Lett
; 9(6): 2402-6, 2009 Jun.
Article
en En
| MEDLINE
| ID: mdl-19422265
ABSTRACT
The safe and effective delivery of RNA therapeutics remains the major barrier to their broad clinical application. Here we develop a new nanoparticulate delivery system based on inorganic particles and biodegradable polycations. First, gold nanoparticles were modified with the hydrophilic polymer poly(ethylene glycol) (PEG), and then small interfering RNA (siRNA) was conjugated to the nanoparticles via biodegradable disulfide linkages, with approximately 30 strands of siRNA per nanoparticle. The particles were then coated with a library of end-modified poly(beta-amino ester)s (PBAEs), previously identified as capable of facilitating intracellular DNA delivery. Nanoparticulate formulations developed here facilitate high levels of in vitro siRNA delivery, facilitating delivery as good or better than the commercially available lipid reagent, Lipofectamine 2000.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
/
Vehículos Farmacéuticos
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ARN Interferente Pequeño
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Nanopartículas del Metal
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Oro
Límite:
Humans
Idioma:
En
Revista:
Nano Lett
Año:
2009
Tipo del documento:
Article