[Expression of iPLA2 in human pancreatic islets and its important role in glucose-stimulated insulin secretion].
Zhonghua Yi Xue Za Zhi
; 89(20): 1426-9, 2009 May 26.
Article
en Zh
| MEDLINE
| ID: mdl-19671340
ABSTRACT
OBJECTIVE:
To assess the role of calcium-independent phospholipase A2 (iPLA2) in human pancreatic islets.METHODS:
The immunohistochemical analysis and Western blot were employed to examine iPLA2 expression in human pancreatic islets. Bromoenol lactone (BEL), a selective inhibitor of iPLA2, was used in a randomized controlled trial to compare its influence to glucose-stimulated insulin secretion.RESULTS:
iPLA2 was expressed predominantly in islet cells co-stained by insulin but was barely detected in the exocrine acinar cells. Western blot results indicated that islet cells expressed an iPLA2-immunoreactive band at the 80 kDa region. Glucose-stimulated insulin secretory response was dramatically reduced in islets pretreated with BEL (0.8285 +/- 0.0803 ng x islet(-1) x h(-1)) as compared with the control (1.2264 +/- 0.0568 ng x islet(-1) x h(-1)) (P < 0.01). BEL inhibited glucose stimulated insulin secretion from isolated human islets.CONCLUSION:
iPLA2 signaling plays an important role in glucose-stimulated insulin secretion under the physiological conditions.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Islotes Pancreáticos
/
Fosfolipasas A2 Calcio-Independiente
/
Insulina
Tipo de estudio:
Clinical_trials
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Yi Xue Za Zhi
Año:
2009
Tipo del documento:
Article
País de afiliación:
China