[Nandeshi: a powerful inhibitor of human acrosin activity].
Zhonghua Nan Ke Xue
; 15(8): 700-2, 2009 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-19852269
ABSTRACT
OBJECTIVE:
To evaluate the inhibitory effect of Nandeshi, an acrosin inhibitor, on human acrosin activity.METHODS:
We collected sperm samples from 10 healthy fertile men and cultured them with Nandeshi at 30 degrees C for 5 minutes at the concentrations of 0. 100, 0.120, 0.144, 0.173, 0.207, 0.249, 0.299, 0.358 and 0.430 mmol/L, with the controls treated with a well-known acrosin inhibitor N-alpha-p-tosyl-L-lysine chloromethylketone (TLCK) at 150.0, 189.8, 213.6, 240.3, 270.3, 304.1 and 342.1 mmol/L. Then we determined the residual activity of human acrosin by improved Kennedy assay.RESULTS:
The residual activity of acrosin was negatively correlated with the Nandeshi concentration, and Nandeshi exhibited an inhibition rate about 800 times that of TLCK.CONCLUSION:
Nandeshi has a powerful inhibitory effect on human acrosin, and improved Kennedy assay is a simple, practical and highly sensitive technique for the detection of human acrosin activity.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Acrosina
/
Anticonceptivos Femeninos
/
Inhibidores Enzimáticos
Límite:
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2009
Tipo del documento:
Article
País de afiliación:
China