Neuroanatomical tracing combined with in situ hybridization: analysis of gene expression patterns within brain circuits of interest.
J Neurosci Methods
; 194(1): 28-33, 2010 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-19961877
ABSTRACT
Most of our current understanding of brain circuits is based on hodological studies carried out using neuroanatomical tract-tracing. Our aim is to advance one step further by visualizing the functional correlate in a given circuit. In this regard, we believe it is feasible to combine retrograde tracing with fluorescence, non-radioactive in situ hybridization (ISH) protocols. The subsequent detection at the single-cell level of the expression of a given mRNA within retrograde-labeled neurons provides information regarding cellular function. This may be of particular interest when trying to elucidate the performance of brain circuits of interest in animal models of brain diseases. Several combinations of retrograde tracing with either single- and double-ISH are presented here, together with some criteria that influence the selection of the tracer to be used in conjunction with the strong demands of the ISH.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Química Encefálica
/
Expresión Génica
/
Hibridación in Situ
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Técnicas de Trazados de Vías Neuroanatómicas
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Vías Nerviosas
/
Neuroanatomía
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Año:
2010
Tipo del documento:
Article
País de afiliación:
España