High-throughput generation of selected reaction-monitoring assays for proteins and proteomes.
Nat Methods
; 7(1): 43-6, 2010 Jan.
Article
en En
| MEDLINE
| ID: mdl-19966807
ABSTRACT
Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Bioensayo
/
Proteínas
/
Biblioteca de Péptidos
/
Proteoma
/
Ensayos Analíticos de Alto Rendimiento
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2010
Tipo del documento:
Article
País de afiliación:
Suiza