High-throughput dynamic light scattering method for measuring viscosity of concentrated protein solutions.
Anal Biochem
; 399(1): 141-3, 2010 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-19995543
ABSTRACT
We propose a new method to measure the viscosity of concentrated protein solutions in a high-throughput format. This method measures the apparent hydrodynamic radius of polystyrene beads with known sizes using a dynamic light scattering (DLS) system with a microplate reader. Glycerol solution viscosities obtained by the DLS method were in good agreement with those reported in the literature. Viscosity of the solutions of two monoclonal antibody molecules was acquired using both DLS and cone-and-plate techniques, and the results were comparable. The DLS method described here has the potential to be used in many aspects of protein characterization.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Dispersión de Radiación
/
Proteínas
/
Luz
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos