Effect of amnion-derived cellular cytokine solution on healing of experimental partial-thickness burns.
World J Surg
; 34(7): 1663-8, 2010 Jul.
Article
en En
| MEDLINE
| ID: mdl-20127332
ABSTRACT
BACKGROUND:
Amnion-derived multipotent progenitor (AMP) cells, unlike most stem cells, have been demonstrated to be nontumorigenic and nonimmunogenic. Amnion-derived cellular cytokine solution (ACCS), a secreted product of AMP cells, is a cocktail of cytokines existing at physiological levels and has been used to accelerate epithelialization of experimental partial-thickness burns.METHODS:
Using modifications of Zawacki's guinea pig partial-thickness scald burn model, a total of 65 animals were treated with ACCS, ACCS + AMP cells, unconditioned medium (UCM) + AMP cells, or either UCM alone or saline as controls. Dosage times ranged from every other day to once a week. Percent epithelialization was serially determined from acetate wound tracings. Histology was performed on wound biopsies.RESULTS:
ACCS, UCM + AMP cells, and ACCS + AMP cells improved epithelialization compared with the two control groups (P < 0.05). When ACCS was delivered more frequently, statistically significant more rapid epithelialization occurred (P < 0.05). By day 7, all groups treated with ACCS had reached at least 90% epithelialization, whereas control groups were only 20-40% epithelialized (P < 0.05). Histology showed excellent regeneration of the epidermis with rete ridge formation. Hair growth occurred in ACCS-treated animals but not in the control group.CONCLUSIONS:
Amnion-derived cellular cytokine solution accelerates the healing of experimental partial-thickness burns. Based on these findings, a multicenter clinical trial is underway.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cicatrización de Heridas
/
Quemaduras
/
Citocinas
/
Células Madre Multipotentes
/
Trasplante de Células Madre
/
Amnios
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
World J Surg
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos