Expression changes of transforming growth factor-beta1 and thrombospondin-1 in cavernous tissues of diabetic rats.
Urol Int
; 84(2): 221-5, 2010.
Article
en En
| MEDLINE
| ID: mdl-20215829
ABSTRACT
OBJECTIVE:
To assess transforming growth factor-beta1 (TGF-beta1) and thrombospondin-1 (TSP-1) expression in the cavernous tissue of rats with streptozotocin (STZ)-induced diabetes. MATERIALS ANDMETHODS:
Twenty male Sprague-Dawley rats were randomly divided into 2 groups diabetics and controls. We injected STZ intraperitoneally to induce diabetes, and studied the alterations in TGF-beta1 and TSP-1 expression in the cavernous tissue of the 2 groups by immunohistochemistry and real-time quantitative reverse transcription polymerase chain reaction. HE staining was also applied to determine morphological changes. Weight, blood sugar and urine sugar were measured before and after model induction in both groups.RESULTS:
Expression of TGF-beta1 and TSP-1 increased significantly in the cavernous tissue of the diabetic rats compared to the control group.CONCLUSIONS:
TGF-beta1 and TSP-1 expression changes in cavernous tissues may play an important role in diabetic erectile dysfunction.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Regulación de la Expresión Génica
/
Trombospondina 1
/
Perfilación de la Expresión Génica
/
Diabetes Mellitus Experimental
/
Factor de Crecimiento Transformador beta1
/
Disfunción Eréctil
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Urol Int
Año:
2010
Tipo del documento:
Article