A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet.
Anal Biochem
; 402(1): 99-101, 2010 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-20230772
ABSTRACT
We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8-1.6 ng of phiX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Colorantes de Rosanilina
/
Coloración y Etiquetado
/
ADN
/
Electroforesis en Gel de Poliacrilamida
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Anal Biochem
Año:
2010
Tipo del documento:
Article
País de afiliación:
China