Identification and characterization of the maize arogenate dehydrogenase gene family.
J Exp Bot
; 61(13): 3663-73, 2010 Aug.
Article
en En
| MEDLINE
| ID: mdl-20558569
ABSTRACT
In plants, the amino acids tyrosine and phenylalanine are synthesized from arogenate by arogenate dehydrogenase and arogenate dehydratase, respectively, with the relative flux to each being tightly controlled. Here the characterization of a maize opaque endosperm mutant (mto140), which also shows retarded vegetative growth, is described The opaque phenotype co-segregates with a Mutator transposon insertion in an arogenate dehydrogenase gene (zmAroDH-1) and this led to the characterization of the four-member family of maize arogenate dehydrogenase genes (zmAroDH-1-zmAroDH-4) which share highly similar sequences. A Mutator insertion at an equivalent position in AroDH-3, the most closely related family member to AroDH-1, is also associated with opaque endosperm and stunted vegetative growth phenotypes. Overlapping but differential expression patterns as well as subtle mutant effects on the accumulation of tyrosine and phenylalanine in endosperm, embryo, and leaf tissues suggest that the functional redundancy of this gene family provides metabolic plasticity for the synthesis of these important amino acids. mto140/arodh-1 seeds shows a general reduction in zein storage protein accumulation and an elevated lysine phenotype typical of other opaque endosperm mutants, but it is distinct because it does not result from quantitative or qualitative defects in the accumulation of specific zeins but rather from a disruption in amino acid biosynthesis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Prefenato Deshidrogenasa
/
Semillas
/
Regulación de la Expresión Génica de las Plantas
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Zea mays
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Idioma:
En
Revista:
J Exp Bot
Asunto de la revista:
BOTANICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos