Novel mucoadhesive polysaccharide isolated from Bletilla striata improves the intraocular penetration and efficacy of levofloxacin in the topical treatment of experimental bacterial keratitis.
J Pharm Pharmacol
; 62(9): 1152-7, 2010 Sep.
Article
en En
| MEDLINE
| ID: mdl-20796194
ABSTRACT
OBJECTIVES:
The objective of the present study was to evaluate a novel mucoadhesive polymer extracted from Bletilla striata for ocular delivery of 0.5% levofloxacin in rabbits, and to determine its improved efficacy against experimental keratitis.METHODS:
B. striata polysaccharide (BsP) was subjected to cell cytotoxicity and ferning tests. The pharmacokinetics and bioavailability of topically applied 0.5% levofloxacin-BsP eye drops was investigated and compared with 0.5% levofloxacin eye drops (Cravit). Experimental Staphylococcus aureus keratitis was induced and treated with levofloxacin or levofloxacin-BsP eye drops. KEYFINDINGS:
BsP markedly increased the proliferative capacity of a human corneal epithelial [corrected] cell line. The ferning test showed that BsP exhibited optimal performance as a tear fluid. The polysaccharides significantly increased intra-aqueous penetration and corneal accumulation in rabbits. Treatment with levofloxacin-BsP reduced the number of organisms more significantly than eye drops containing levofloxacin alone.CONCLUSIONS:
BsP appears to be a promising candidate as a vehicle for topical ophthalmic drug delivery, especially for antibiotics.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Infecciones Estafilocócicas
/
Extractos Vegetales
/
Ofloxacino
/
Orchidaceae
/
Levofloxacino
/
Queratitis
/
Antibacterianos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Female
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Humans
/
Male
Idioma:
En
Revista:
J Pharm Pharmacol
Año:
2010
Tipo del documento:
Article