Solution structure of the glucocorticoid receptor DNA-binding domain.
Science
; 249(4965): 157-60, 1990 Jul 13.
Article
en En
| MEDLINE
| ID: mdl-2115209
ABSTRACT
The three-dimensional structure of the DNA-binding domain (DBD) of the glucocorticoid receptor has been determined by nuclear magnetic resonance spectroscopy and distance geometry. The structure of a 71-residue protein fragment containing two "zinc finger" domains is based on a large set of proton-proton distances derived from nuclear Overhauser enhancement spectra, hydrogen bonds in previously identified secondary structure elements, and coordination of two zinc atoms by conserved cysteine residues. The DBD is found to consist of a globular body from which the finger regions extend. A model of the dimeric complex between the DBD and the glucocorticoid response element is proposed. The model is consistent with previous results indicating that specific amino acid residues of the DBD are involved in protein-DNA and protein-protein interactions.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Receptores de Glucocorticoides
/
Proteínas de Unión al ADN
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Science
Año:
1990
Tipo del documento:
Article
País de afiliación:
Países Bajos