PML protein analysis using imaging flow cytometry.
J Clin Pathol
; 64(5): 447-50, 2011 May.
Article
en En
| MEDLINE
| ID: mdl-21177751
ABSTRACT
Acute promyelocytic leukaemia (APML) can be promptly diagnosed by detecting abnormal diffuse staining patterns of PML bodies in abnormal promyelocytes using immunofluorescence microscopy. However, this technique is subjective, with low sensitivity. Using samples from 18 patients with acute myeloid leukaemia (AML) (including four with APML), the authors investigated whether imaging flow cytometry could be a viable alternative to this current technique and improve sensitivity levels. Bone marrow/peripheral blood cells were stained with an antibody to PML, and data were acquired on an ImageStream (Amnis Corporation, Seattle, Washington, USA). Using the modulation feature for data analysis, the authors demonstrated that this technique could successfully identify cases of APML. Imaging flow cytometry, by analysing greater numbers of cells and with the potential to include disease-specific antigens, increases the sensitivity of the current immunofluorescence technique. Imaging flow cytometry is an exciting technology that has many possible applications in the diagnosis of haematological malignancies, including the potential to integrate modalities.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Factores de Transcripción
/
Proteínas Nucleares
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Leucemia Mieloide Aguda
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Proteínas Supresoras de Tumor
/
Citometría de Flujo
Tipo de estudio:
Prognostic_studies
Límite:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Humans
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Middle aged
Idioma:
En
Revista:
J Clin Pathol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Reino Unido