Detection of low-level K65R variants in nucleoside reverse transcriptase inhibitor-naive chronic and acute HIV-1 subtype C infections.
J Infect Dis
; 203(6): 798-802, 2011 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-21257741
ABSTRACT
To substantiate reports of greater emergence of the K65R nucleoside reverse transcriptase inhibitor (NRTI) mutation in human immunodeficiency virus type 1 (HIV-1) subtype C, we examined natural low-level K65R expression in subtype C relative to subtypes B and AE. We used allele-specific polymerase chain reaction to screen HIV-1 amplified by reverse-transcription high-fidelity polymerase chain reaction from subtype C-infected South African women and infants and CRF01(subtype AE) from Thailand; all subjects were NRTI naive. We found low-level K65R of unknown clinical significance in NRTI-naive subtype C-infected women and infants at frequencies above the natural occurrence in subtypes B and AE. The frequent appearance of subtype C frameshift deletions at codon 65 supports a propensity for transcription error in this region.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Infecciones por VIH
/
VIH-1
/
ADN Polimerasa Dirigida por ARN
/
Inhibidores de la Transcriptasa Inversa
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
Límite:
Female
/
Humans
/
Infant
/
Newborn
/
Pregnancy
País/Región como asunto:
Africa
/
Asia
Idioma:
En
Revista:
J Infect Dis
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos