Identification of a sequence in the matricellular protein SPARC that interacts with the scavenger receptor stabilin-1.
J Cell Biochem
; 112(4): 1003-8, 2011 Apr.
Article
en En
| MEDLINE
| ID: mdl-21308731
ABSTRACT
SPARC (osteonectin/BM-40), a secreted matricellular protein that promotes cellular deadhesion and motility in wound healing, carcinogenesis, and inflammation, binds to the scavenger receptor stabilin-1 in alternatively activated macrophages and undergoes endocytosis and clearance from the extracellular space. Both SPARC and stabilin-1 are expressed by endothelial cells during inflammation, but their interaction in this context is unknown. We have identified a binding site on SPARC for stabilin-1 by a solid-state peptide array coupled with a modified enzyme-linked immunosorbent assay. A monoclonal antibody that recognizes the identified binding site was also characterized that could be an inhibitor for the SPARC-stabilin-1 interaction in macrophages or endothelial cells.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Moléculas de Adhesión Celular Neuronal
/
Osteonectina
/
Receptores Mensajeros de Linfocitos
/
Epítopos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Cell Biochem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos