Detection of single-base mutation in RNA using T4 RNA ligase-based nick-joining or DNAzyme-based nick-generation.
Anal Biochem
; 414(2): 303-5, 2011 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-21453671
ABSTRACT
A single nucleotide polymorphism (SNP) is a common genetic variation when a single nucleotide differs between members of a species or paired chromosome. Due to its association with disease susceptibility and drug resistance, SNP detection is of great value in studying the variation in drug responses. Here we present two quantitative SNP detection methods for a single-base mismatch in RNA, based on nick-joining and nick-generating activities of T4 RNA ligase and DNAzyme, respectively. T4 RNA ligase successfully discriminated a one-base mismatch in the ligation junction, and the designed DNAzyme cleaved RNA by discerning a single-base mismatch in the cleaving site.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN Ligasa (ATP)
/
Proteínas Virales
/
ARN
/
Técnicas de Sonda Molecular
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Disparidad de Par Base
/
ADN Catalítico
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Biochem
Año:
2011
Tipo del documento:
Article