Unlocking the barley genome by chromosomal and comparative genomics.
Plant Cell
; 23(4): 1249-63, 2011 Apr.
Article
en En
| MEDLINE
| ID: mdl-21467582
ABSTRACT
We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign ~86% of the estimated ~32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hordeum
/
Genoma de Planta
/
Genómica
/
Cromosomas de las Plantas
Idioma:
En
Revista:
Plant Cell
Asunto de la revista:
BOTANICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania