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Discovery of DNA operators for TetR and MarR family transcription factors from Burkholderia xenovorans.
Maity, Tuhin Subhra; Close, Devin W; Valdez, Yolanda E; Nowak-Lovato, Kristy; Marti-Arbona, Ricardo; Nguyen, Tinh T; Unkefer, Pat J; Hong-Geller, Elizabeth; Bradbury, Andrew R M; Dunbar, John.
Afiliación
  • Maity TS; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Close DW; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Valdez YE; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Nowak-Lovato K; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Marti-Arbona R; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Nguyen TT; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Unkefer PJ; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Hong-Geller E; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Bradbury ARM; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Dunbar J; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Microbiology (Reading) ; 158(Pt 2): 571-582, 2012 Feb.
Article en En | MEDLINE | ID: mdl-22117006
ABSTRACT
Determining transcription factor (TF) recognition motifs or operator sites is central to understanding gene regulation, yet few operators have been characterized. In this study, we used a protein-binding microarray (PBM) to discover the DNA recognition sites and putative regulons for three TetR and one MarR family TFs derived from Burkholderia xenovorans, which are common to the genus Burkholderia. We also describe the development and application of a more streamlined version of the PBM technology that significantly reduced the experimental time. Despite the genus containing many pathogenically important species, only a handful of TF operator sites have been experimentally characterized for Burkholderia to date. Our study provides a significant addition to this knowledge base and illustrates some general challenges of discovering operators on a large scale for prokaryotes.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Factores de Transcripción / Regiones Operadoras Genéticas / Burkholderia Idioma: En Revista: Microbiology (Reading) Asunto de la revista: MICROBIOLOGIA Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Factores de Transcripción / Regiones Operadoras Genéticas / Burkholderia Idioma: En Revista: Microbiology (Reading) Asunto de la revista: MICROBIOLOGIA Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos