[Pollen germination in vitro and pollen tube growth of Scutellaria baicalensis].

ABSTRACT

OBJECTIVE: To study the pollen germination in vitro and pollen tube growth of Scutellaria baicalensis. METHOD: Two years-old S. baicalensis that in the experiment field of Shangluo university were used as the materials. The effects of culture medium, sucrose, PEG, pH value, culture time and temperature on the pollen germination and tube growth of S. baicalensis were investigated using the method of liquid culture. RESULT AND CONCLUSION: The results showed that 100 g x L(-1) sucrose was helpful for the germination and growth of pollens. However, higher concentration of sucrose would inhibit strongly pollen germination and the growth of pollen tubes. PEG could accelerate pollen germination and tube growth, and the optimal concentration was 150 g x L(-1). The optimum culture medium was BK and BK + 10% sucrose + 150 g x L(-1) PEG (pH 5.8) , and the latter was more benefit for pollen germination and tube growth. The pollen of germination was better at 25-35 degrees C, and it was the best at 30 degrees C for 2-3 hours, while pollen tube growth was slower at 25 degrees C.