Flow-injection chemiluminescence determination of haemoglobin in the blood.
Luminescence
; 28(1): 56-62, 2013.
Article
en En
| MEDLINE
| ID: mdl-22298437
ABSTRACT
In this study, a sensitive and simple flow-injection chemiluminescence (CL) method was developed for the quantitative analysis of haemoglobin. The method is based on the ability of haemoglobin to enhance the CL signal generated by a H(2)O(2) -K(4) Fe(CN)(6) -fluorescein alkaline system enhanced by CdTe quantum dots. Under the optimized conditions, haemoglobin can be detected in concentration range 7.35 × 10(-9) -2.5 × 10(-6) mol/L, with a detection limit (3σ) of 1.8 × 10(-9) mol/L and a relative standard deviation (RSD; for 5 × 10(-7) mol/L haemoglobin) of 2.06% (n = 11). The present CL method was successfully applied for the determination of haemoglobin in three kinds of blood samples taken from an infant, an adult man, an adult woman and two reference samples. Compared with previous reports, the CL method described in this work is simple and rapid, with high sensitivity.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hemoglobinas
/
Análisis de Inyección de Flujo
/
Mediciones Luminiscentes
Tipo de estudio:
Evaluation_studies
Límite:
Adult
/
Female
/
Humans
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Male
/
Newborn
Idioma:
En
Revista:
Luminescence
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
2013
Tipo del documento:
Article