CUX1 transcription factor is required for optimal ATM/ATR-mediated responses to DNA damage.
Nucleic Acids Res
; 40(10): 4483-95, 2012 May.
Article
en En
| MEDLINE
| ID: mdl-22319212
ABSTRACT
The p110 Cut homeobox 1 (CUX1) transcription factor regulates genes involved in DNA replication and chromosome segregation. Using a genome-wide-approach, we now demonstrate that CUX1 also modulates the constitutive expression of DNA damage response genes, including ones encoding ATM and ATR, as well as proteins involved in DNA damage-induced activation of, and signaling through, these kinases. Consistently, RNAi knockdown or genetic inactivation of CUX1 reduced ATM/ATR expression and negatively impacted hallmark protective responses mediated by ATM and ATR following exposure to ionizing radiation (IR) and UV, respectively. Specifically, abrogation of CUX1 strongly reduced ATM autophosphorylation after IR, in turn causing substantial decreases in (i) levels of phospho-Chk2 and p53, (ii) γ-H2AX and Rad51 DNA damage foci and (iii) the efficiency of DNA strand break repair. Similarly remarkable reductions in ATR-dependent responses, including phosphorylation of Chk1 and H2AX, were observed post-UV. Finally, multiple cell cycle checkpoints and clonogenic survival were compromised in CUX1 knockdown cells. Our results indicate that CUX1 regulates a transcriptional program that is necessary to mount an efficient response to mutagenic insult. Thus, CUX1 ensures not only the proper duplication and segregation of the genetic material, but also the preservation of its integrity.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
/
Daño del ADN
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Proteínas Nucleares
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Proteínas Serina-Treonina Quinasas
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Proteínas de Homeodominio
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Proteínas de Ciclo Celular
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Proteínas Supresoras de Tumor
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Proteínas de Unión al ADN
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Reparación del ADN
Límite:
Animals
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Humans
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2012
Tipo del documento:
Article