The effect of edaravone on ischemia-reperfusion injury in rat ovary.

ABSTRACT

OBJECTIVE: The aim of this study was to investigate the effect of edaravone on experimentally induced ovarian torsion/detorsion ischemia/reperfusion (I/R) injury. STUDY DESIGN: Forty-six female adult Wistar-Albino rats were utilized to create five groups In group 1, only 5 mg/kg edaravone was given and ovary torsion was not performed. In group 2, torsion was not performed and no drug was given. In group 3, vehicle was given and torsion/detorsion was performed. In group 4, 1 mg/kg edaravone was given and torsion/detorsion was performed. In group 5, edaravone; 5 mg/kg drug was administered and torsion/detorsion was performed. Right ovarian torsion was simulated for a 3-h period of ischemia and a 1-h reperfusion period. Right ovaries were then surgically extirpated in all groups. In ovarian tissue samples malondialdehyde (MDA) levels and activity of superoxide dismutase were studied. Microscopic ovarian tissue damage was scored by histologic and electron microscopic findings. RESULTS: The MDA level in the group 5 was significantly lower than group 3 (p<0.001). Superoxide dismutase activity in the group 5 was significantly higher than group 3 (p<0.001). Histopathological ovarian tissue damage in the group 5 were significantly lower than group 3 (p<0.001). CONCLUSION: These results indicate that edaravone could be an effective agent in the short-term treatment and prevention of ovarian ischemia and reperfusion damage.