Antibiotic susceptibility pattern and the indicator of decreased ciprofloxacin susceptibility of Salmonella enterica serovar Typhi isolated from Dhulikhel Hospital, Nepal.
Jpn J Infect Dis
; 65(3): 264-7, 2012.
Article
en En
| MEDLINE
| ID: mdl-22627312
ABSTRACT
Monitoring the antibiotic susceptibility pattern of Salmonella enterica serovar Typhi (S. Typhi) is important for efficiently managing cases of typhoid fever. In this study, the antimicrobial susceptibility patterns of 114 S. Typhi isolates, which were collected from a university hospital in Nepal during July 2009-December 2010, were investigated by disc diffusion assays. All of the S. Typhi isolates were sensitive to amoxycillin-clavulanic acid. More than 95% of the isolates were sensitive to chloramphenicol, ceftazidime, ceftriaxone, and cotrimoxazole. In addition, 1.7% of the studied isolates showed multiple drug resistance patterns. Of the 40 S. Typhi isolates, 32 strains (80%) showed nalidixic acid (NA) resistance with decreased susceptibility to ciprofloxacin (CIP). Importantly, we found the simultaneous presence of NA resistance and decreased susceptibility to CIP, suggesting that the resistance to NA is a reliable indicator of decreased CIP susceptibility (sensitivity, 97.5%; specificity, 100.0%). Furthermore, the sequencing of NA-resistant S. Typhi isolates showed a predominant amino acid alteration in the quinolone resistance-determining region (QRDR) of gyrA gene at position 83 from SerâPhe. Two isolates with resistance to both CIP and NA had a double-mutation (Ser83âPhe and Asp87âAsn) in the QRDR of the gyrA gene, of which one had an additional amino acid mutation (Ser80âIlu) in the QRDR of the parC gene.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Salmonella typhi
/
Fiebre Tifoidea
/
Ciprofloxacina
/
Farmacorresistencia Bacteriana
/
Hospitales Universitarios
/
Antibacterianos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Jpn J Infect Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
Año:
2012
Tipo del documento:
Article
País de afiliación:
Tailandia