Simultaneous optically sectioned fluorescence and optical coherence microscopy with full-field illumination.
Opt Lett
; 37(10): 1613-5, 2012 May 15.
Article
en En
| MEDLINE
| ID: mdl-22627513
ABSTRACT
Full-field optical coherence microscopy (FF-OCM) and optically sectioned fluorescence microscopy are two imaging techniques that are implemented here in a novel dual modality instrument. The two imaging modalities use a broad field illumination to acquire the entire field of view without raster scanning. Optical sectioning is achieved in both imaging modalities owing to the coherence gating property of light for FF-OCM, and a structured illumination setup for fluorescence microscopy. Complementary image data are provided by the dual modality instrument in the context of biological tissue screening. FF-OCM imaging modality shows the tissue microarchitecture, while fluorescence microscopy highlights specific tissue features with cellular-level resolution by using targeting contrast agents. Complementary tissue morphology and biochemical features could potentially improve the understanding of cellular functions and disease diagnosis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Iluminación
/
Tomografía de Coherencia Óptica
/
Fenómenos Ópticos
/
Microscopía Fluorescente
Límite:
Animals
Idioma:
En
Revista:
Opt Lett
Año:
2012
Tipo del documento:
Article
País de afiliación:
Francia