[Effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines contents of IEC-6 cell].
Zhong Yao Cai
; 35(7): 1112-6, 2012 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-23252278
ABSTRACT
OBJECTIVE:
To study the effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines (putrescine, spermidine and spermine) contents of IEC-6 cell.METHODS:
Cell migration model was induced by scratch method in each well,and the polyamines in IEC-6 cell was determined by pre-column derivation high performance liquid chromatography. The polysaccharides inhibited effect on migration and polyamines contents of IEC-6 cells, and on IEC-6 cell migration by DFMO (a polyamines synthesis inhibitor) and the polyamines contents in the cells were observed.RESULTS:
The polysaccharides (50 mg/L or 100 mg/L) was able to promote the cell migration, reverse the cell migration inhibition by DFMO, enhance the IEC-6 cell polyamines (putrescine, spermidine and spermine) contents in the process of cell migration and reverse the reduction of polyamines (putrescine, spermidine and spermine) induced by DFMO.CONCLUSION:
The effect of Radix Glycyrrhizae on the gastrointestinal mucosal damage repairing may be related to increasing polyamine content in cells and promoting cell migration.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Poliaminas
/
Polisacáridos
/
Células Epiteliales
/
Glycyrrhiza
/
Mucosa Intestinal
Límite:
Animals
Idioma:
Zh
Revista:
Zhong Yao Cai
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2012
Tipo del documento:
Article
País de afiliación:
China