Controlled delivery of low-dose bone morphogenetic protein-2 using heparin-conjugated fibrin in the posterolateral lumbar fusion of rabbits.
Artif Organs
; 37(5): 487-94, 2013 May.
Article
en En
| MEDLINE
| ID: mdl-23461810
The heparin-conjugated fibrin (HCF) system has been developed to deliver bone morphogenetic proteins (BMPs) for a long-term period and thus enhance bone regeneration. In the present study, we tested the effectiveness of the delivery system for spinal fusion with a very low dose of BMP-2. A total of 15 rabbits underwent posterolateral lumbar spine, divided into three groups. The control group received only collagen sponges without BMP-2, another group (BMP-only group) received collagen sponges loaded with BMP-2 (10 µg each side), and the last group (BMP/HCF group) received collagen sponges filled with HCF loaded with BMP-2 (10 µg each side). All animals were euthanized 8 weeks after surgery, and the fusion was assessed by radiographs, manual palpation, computed tomography scan, and mechanical testing. No case in the BMP/HCF group or in the control group achieved solid fusion, while all cases in BMP-only group showed evidence of solid fusion. BMP/HCF group had significantly lower fusion rate and tensile strength than BMP-only group at the dose of 10 µg of BMP-2. The HCF long-term delivery system with the low dose of BMP-2 (10 µg) is ineffective for the induction of lumbar posterolateral fusion in the rabbits.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fusión Vertebral
/
Regeneración Ósea
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Fibrina
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Portadores de Fármacos
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Heparina
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Tapones Quirúrgicos de Gaza
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Proteína Morfogenética Ósea 2
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Vértebras Lumbares
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Artif Organs
Año:
2013
Tipo del documento:
Article