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UV irradiation study of a tripeptide isolated in an argon matrix: a tautomerism process evidenced by infrared and X-ray photoemission spectroscopies.
Mateo-Marti, E; Pradier, C M.
Afiliación
  • Mateo-Marti E; Centro de Astrobiología (CSIC-INTA), Torrejón de Ardoz, Madrid, Spain. mateome@cab.inta-csic.es
Spectrochim Acta A Mol Biomol Spectrosc ; 109: 247-52, 2013 May 15.
Article en En | MEDLINE | ID: mdl-23542515
ABSTRACT
Matrix isolation is a powerful tool for studying photochemical processes occurring in isolated molecules. In this way, we characterized the chemical modifications occurring within a tri peptide molecule, IGF, when exposed to the influence of Ultraviolet (UV) irradiation. This paper first describes the successful formation of the tripeptide (IGF) argon matrix under vacuum conditions, followed by the in situ UV irradiation and characterization of the molecular matrix reactivity after UV-irradiation. These studies have been performed by combining two complementary spectroscopic techniques, Fourier-Transform Reflexion Absorption Spectroscopy (FT-IRRAS) and X-ray Photoelectron Spectroscopy (XPS). The IR spectra of the isolated peptide-matrix, before and after UV irradiation, revealed significant differences that could be associated either to a partial deprotonation of the molecule or to a tautomeric conversion of some amide bonds to imide ones on some peptide molecules. XPS analyses undoubtedly confirmed the second hypothesis; the combination of IRRAS and XPS results provide evidence that UV irradiation of peptides induces a chemical reaction, namely a shift of the double bond, meaning partial conversion from amide tautomer into an imidic acid tautomer.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Argón / Factor I del Crecimiento Similar a la Insulina / Factor II del Crecimiento Similar a la Insulina Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2013 Tipo del documento: Article País de afiliación: España

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Argón / Factor I del Crecimiento Similar a la Insulina / Factor II del Crecimiento Similar a la Insulina Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2013 Tipo del documento: Article País de afiliación: España