Optimization of the fine particle fraction of a lyophilized lysozyme formulation for dry powder inhalation.
Pharm Res
; 30(6): 1698-713, 2013 Jun.
Article
en En
| MEDLINE
| ID: mdl-23568518
ABSTRACT
PURPOSE:
A new dry powder inhalation technology creates inhalable particles from a coherent lyophilized bulk at the time of inhalation. The aim of this study was to evaluate several approaches to improve the fine particle fraction (FPF) and to understand underlying mechanisms.METHODS:
Lysozyme was chosen as model drug. Phenylalanine and valine were added, and the freezing process was varied. Lyophilisate characteristics as well as aerosolization behavior was analyzed.RESULTS:
The addition of the crystalline amino acids rendered a dose independent three-fold increase of the FPF. This is possibly due to enhanced fracture properties of the lyophilisates upon impact of the air stream and reduced particle agglomeration/cohesion caused by a rougher surface. This positive effect was well preserved over 3 months of storage. The structure of the lyophilisate was influenced by the freezing process which in turn affected the aerosolization behavior. Liquid nitrogen and vacuum-induced freezing performed best, doubling the FPF. The special cake morphology with elongated channels enabled easy disintegration. The resulting large porous particles comprise a low density being advantageous for a high FPF.CONCLUSION:
The variation of the lyophilization process and formulation utilizing excipients enabled an optimization of the FPF of the novel lyophilisate based DPI system.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Muramidasa
/
Química Farmacéutica
/
Inhaladores de Polvo Seco
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Pharm Res
Año:
2013
Tipo del documento:
Article
País de afiliación:
Alemania