The phosphatidylcholine-transfer protein catalyzed import of phosphatidylcholine into isolated rat liver mitochondria.
Biochim Biophys Acta
; 1025(1): 49-59, 1990 Jun 11.
Article
en En
| MEDLINE
| ID: mdl-2369576
ABSTRACT
In order to study the individual steps involved in the import of phosphatidylcholine (PC) into rat liver mitochondria, a number of PC analogues were introduced into the outer membrane of isolated mitochondria. Two fluorescent PC species, i.e. 1-palmitoyl-2-(16-bimanylthio)hexadecanoyl-PC (bimane-PC) and 1-palmitoyl-2-(10-pyrene)decanoyl-PC (pyrene-PC), and one radiolabeled PC species, i.e. 1-palmitoyl-2-[1-14C]oleoyl-PC (14C-POPC), were studied. The PC analogues were introduced from small unilamellar vesicles with the use of PC-specific transfer protein. The amount of PC imported was quantified by reisolation of the mitochondria. Import of the fluorescent PC species was monitored by on-line fluorescence spectroscopy. The distribution of the newly inserted PC between the outer and the inner membrane was assessed by separation of the two membranes using digitonin treatment. All analogues tested remained exclusively localized in the outer membrane thereby suggesting that additional (extramitochondrial) factors are required to initiate transfer of PC to the inner membrane.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfatidilcolinas
/
Mitocondrias Hepáticas
/
Proteínas Portadoras
/
Proteína de Unión a Andrógenos
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1990
Tipo del documento:
Article
País de afiliación:
Países Bajos