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Age-dependent alteration of intraocular soluble heparan sulfate levels and its implications for proliferative diabetic retinopathy.
Nishiguchi, Koji M; Ushida, Hiroaki; Tomida, Daisuke; Kachi, Shu; Kondo, Mineo; Terasaki, Hiroko.
Afiliación
  • Nishiguchi KM; Department of Ophthalmology, Nagoya University School of Medicine, Showa-ku, Nagoya, Japan. knishiguchi@ucl.ac.uk
Mol Vis ; 19: 1125-31, 2013.
Article en En | MEDLINE | ID: mdl-23734081
ABSTRACT

PURPOSE:

To assess the relationship between intraocular soluble heparan sulfate (HS) concentration and age in subjects with and without diabetic retinopathy.

METHODS:

Vitreous from subjects with idiopathic maculopathies (n=17), i.e., macula hole or epiretinal membrane, or nonproliferative diabetic retinopathy (non-PDR; n=5) and aqueous humor from subjects with PDR (n=16), non-PDR (n=7), or cataracts (n=15) was collected. The levels of HS and vascular endothelial growth factor (VEGF) were measured using enzyme-linked immunosorbent assay. Concentrations of sulfated glycosaminoglycan were determined through dimethylmethylene blue-based assay. The effect of the vitreal HS level on the binding of exogenous VEGF to surface-bound heparin was determined in vitro.

RESULTS:

The level of HS in vitreous samples from subjects with idiopathic maculopathies increased concomitantly with age (p=0.020, R²=0.327). Meanwhile, HS levels in aqueous humor were lower in PDR subjects than in non-PDR (p=0.003) and cataract subjects (p=0.007). However, the PDR subjects were significantly younger than the non-PDR subjects (p<0.001) or cataract subjects (p<0.001). When the three groups were controlled for age, the levels of HS glycosaminoglycans were no longer different between the three (p=0.247). The increasing level of HS or sulfated glycosaminoglycan in the vitreous was associated with its increased inhibitory effect on interaction between VEGF and surface heparin in vitro (p=0.014, R²=0.377).

CONCLUSIONS:

The HS level of the intraocular fluid increased with age. The possible link between low HS in intraocular fluid and increased localization of VEGF at the retinal surface may provide one explanation for the higher susceptibility of younger subjects with diabetes mellitus to developing PDR.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Humor Acuoso / Cuerpo Vítreo / Envejecimiento / Retinopatía Diabética / Heparitina Sulfato Límite: Adult / Aged / Aged80 / Humans / Middle aged Idioma: En Revista: Mol Vis Asunto de la revista: BIOLOGIA MOLECULAR / OFTALMOLOGIA Año: 2013 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Humor Acuoso / Cuerpo Vítreo / Envejecimiento / Retinopatía Diabética / Heparitina Sulfato Límite: Adult / Aged / Aged80 / Humans / Middle aged Idioma: En Revista: Mol Vis Asunto de la revista: BIOLOGIA MOLECULAR / OFTALMOLOGIA Año: 2013 Tipo del documento: Article País de afiliación: Japón