Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences.
Gene
; 528(2): 206-15, 2013 Oct 10.
Article
en En
| MEDLINE
| ID: mdl-23867856
ABSTRACT
An effective DNA marker for authenticating the genus Salvia was screened using seven DNA regions (rbcL, matK, trnL-F, and psbA-trnH from the chloroplast genome, and ITS, ITS1, and ITS2 from the nuclear genome) and three combinations (rbcL+matK, psbA-trnH+ITS1, and trnL-F+ITS1). The present study collected 232 sequences from 27 Salvia species through DNA sequencing and 77 sequences within the same taxa from the GenBank. The discriminatory capabilities of these regions were evaluated in terms of PCR amplification success, intraspecific and interspecific divergence, DNA barcoding gaps, and identification efficiency via a tree-based method. ITS1 was superior to the other marker for discriminating between species, with an accuracy of 81.48%. The three combinations did not increase species discrimination. Finally, we found that ITS1 is a powerful barcode for identifying Salvia species, especially Salvia miltiorrhiza.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN Ribosómico
/
ADN de Cloroplastos
/
Salvia
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Gene
Año:
2013
Tipo del documento:
Article
País de afiliación:
China