Semi-quantitative colony immunoassay for determining and optimizing protein expression in Saccharomyces cerevisiae and Escherichia coli.

Cridge, Andrew G; Visweswaraiah, Jyothsna; Ramesh, Rashmi; Sattlegger, Evelyn

Cridge, Andrew G; Visweswaraiah, Jyothsna; Ramesh, Rashmi; Sattlegger, Evelyn.

Afiliación

Cridge AG; Institute of Natural and Mathematical Sciences, Massey University, Auckland 0745, New Zealand.
Visweswaraiah J; Institute of Natural and Mathematical Sciences, Massey University, Auckland 0745, New Zealand.
Ramesh R; Institute of Natural and Mathematical Sciences, Massey University, Auckland 0745, New Zealand.
Sattlegger E; Institute of Natural and Mathematical Sciences, Massey University, Auckland 0745, New Zealand. Electronic address: e.sattlegger@massey.ac.nz.

Anal Biochem ; 447: 82-9, 2014 Feb 15.

Article en En | MEDLINE | ID: mdl-24176934

Asunto(s)

Escherichia coli/genética; Inmunoensayo/métodos; Proteínas Recombinantes/genética; Saccharomyces cerevisiae/genética; Escherichia coli/citología; Expresión Génica; Ingeniería Genética; Saccharomyces cerevisiae/citología

Palabras clave

Colony Western; Colony expression screening; Colony immunoblotting; Escherichia coli; Protein overexpression; Saccharomyces cerevisiae

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Proteínas Recombinantes / Inmunoensayo / Escherichia coli Idioma: En Revista: Anal Biochem Año: 2014 Tipo del documento: Article País de afiliación: Nueva Zelanda

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