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Role of C596 in the C-terminal extension of the haemagglutinin-neuraminidase protein in replication and pathogenicity of a highly virulent Indonesian strain of Newcastle disease virus.
Kim, Shin-Hee; Xiao, Sa; Paldurai, Anandan; Collins, Peter L; Samal, Siba K.
Afiliación
  • Kim SH; Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, USA.
  • Xiao S; Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, USA.
  • Paldurai A; Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, USA.
  • Collins PL; Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
  • Samal SK; Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, USA.
J Gen Virol ; 95(Pt 2): 331-336, 2014 Feb.
Article en En | MEDLINE | ID: mdl-24197534
We modified the haemagglutinin-neuraminidase (HN) glycoprotein of the virulent Newcastle disease virus (NDV) strain Banjarmasin/010/10 (Ban/010) by adding C-terminal extensions similar to those found in certain avirulent NDV strains. Extension of the 571 aa wt Ban/010 HN protein to 577 and 616 aa by removal of one or two translational stop codons moderately reduced HN function and viral pathogenicity in 1-day-old and 3-week-old chickens. Substantially greater reductions were achieved by altering the 616 aa form by introducing a R596C mutation or by replacing the C-terminal extension with that of avirulent strain Ulster, which naturally contains the amino acid 596C. These results showed that extension of the C terminus of HN reduces NDV pathogenicity, and that this effect is substantially increased by the presence of 596C. These results indicate that this attenuating mechanism in avirulent strains such as Ulster can be applied directly to a highly virulent strain recently in circulation.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Replicación Viral / Virus de la Enfermedad de Newcastle / Proteína HN Límite: Animals Idioma: En Revista: J Gen Virol Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Replicación Viral / Virus de la Enfermedad de Newcastle / Proteína HN Límite: Animals Idioma: En Revista: J Gen Virol Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos