Use of PKA-mediated phenotypes for genetic and small-molecule screens in Schizosaccharomyces pombe.
Biochem Soc Trans
; 41(6): 1692-5, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-24256276
ABSTRACT
PKA (protein kinase A) in the fission yeast Schizosaccharomyces pombe controls transcription of genes involved in metabolism, cell growth and sexual development. In the present review, we discuss phenotypes associated with either high or low PKA activity in the context of how they can be used to carry out genetic or small-molecule screens that affect components of the PKA pathway. Although our recent research has focused on the study of heterologously expressed cyclic nucleotide PDEs (phosphodiesterases), these same methods can be used to target other S. pombe proteins or their functionally equivalent orthologues that act in the PKA pathway.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fenotipo
/
Schizosaccharomyces
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Proteínas Quinasas Dependientes de AMP Cíclico
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Proteínas de Schizosaccharomyces pombe
Idioma:
En
Revista:
Biochem Soc Trans
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos