Multimer monitoring of CMV-specific T cells in research and in clinical applications.
Diagn Microbiol Infect Dis
; 78(3): 201-12, 2014 Mar.
Article
en En
| MEDLINE
| ID: mdl-24331953
ABSTRACT
Multimer monitoring has become a standard technique for detection of antigen-specific T cells. The term "multimer" refers to a group of reagents based on the multimerisation of molecules in order to raise avidity and thus stabilize binding to their ligand. Multimers for detection of antigen-specific T-cell responses are based on major histocompatibility complex class I peptide complexes. Multimer staining enables fast and direct visualization of antigen-specific T cells; thus, it is widely applied to assess antiviral immunity, e.g., monitor patients in vaccination trials or confirm purity of cell products for adoptive transfer. Assessment of T-cell immunity against persistent pathogens like cytomegalovirus (CMV) is of major importance in immunosuppressed patients. Recent advancements of multimers facilitate reversible labeling and allow isolation of epitope-specific T cells for adoptive transfer. Here, we give an overview on the different multimers and their applications, with an emphasis on CMV-specific T-cell responses.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Epítopos de Linfocito T
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Citomegalovirus
/
Multimerización de Proteína
Límite:
Humans
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2014
Tipo del documento:
Article