Targeting RNA-protein interactions within the human immunodeficiency virus type 1 lifecycle.
Biochemistry
; 52(51): 9269-74, 2013 Dec 23.
Article
en En
| MEDLINE
| ID: mdl-24358934
ABSTRACT
RNA-protein interactions are vital throughout the HIV-1 life cycle for the successful production of infectious virus particles. One such essential RNA-protein interaction occurs between the full-length genomic viral RNA and the major structural protein of the virus. The initial interaction is between the Gag polyprotein and the viral RNA packaging signal (psi or Ψ), a highly conserved RNA structural element within the 5'-UTR of the HIV-1 genome, which has gained attention as a potential therapeutic target. Here, we report the application of a target-based assay to identify small molecules, which modulate the interaction between Gag and Ψ. We then demonstrate that one such molecule exhibits potent inhibitory activity in a viral replication assay. The mode of binding of the lead molecules to the RNA target was characterized by ¹H NMR spectroscopy.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ribonucleoproteínas
/
Replicación Viral
/
ARN Viral
/
VIH-1
/
Fármacos Anti-VIH
/
ARN Lider Empalmado
/
Productos del Gen gag del Virus de la Inmunodeficiencia Humana
Límite:
Humans
Idioma:
En
Revista:
Biochemistry
Año:
2013
Tipo del documento:
Article
País de afiliación:
Reino Unido