Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry.
J Clin Microbiol
; 52(4): 1217-9, 2014 Apr.
Article
en En
| MEDLINE
| ID: mdl-24430451
ABSTRACT
PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Peritonitis
/
Bacterias
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Infecciones Bacterianas
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Candida
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Candidiasis
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Soluciones para Diálisis
/
Técnicas Microbiológicas
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Límite:
Adult
/
Aged
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Child
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Clin Microbiol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Taiwán