Subcellular localization of intracellular forms of human chorionic gonadotropin in first trimester placenta.
J Biochem
; 102(5): 993-1001, 1987 Nov.
Article
en En
| MEDLINE
| ID: mdl-2449427
ABSTRACT
To determine the subcellular sites for synthesis and processing of human chorionic gonadotropin subunits in cells, first trimester placental cells were fractionated subcellularly on sucrose density gradients. Analysis of the subcellular fractions by immunobinding techniques revealed that the rough endoplasmic reticulum-rich fraction contained only intermediates having high-mannose oligosaccharides, but the Golgi-rich fraction contained not only intermediates but also mature forms which were resistant to endoglycosidase H but sensitive to neuraminidase. These results show that human chorionic gonadotropin subunits are synthesized in the rough endoplasmic reticulum as forms containing high-mannose oligosaccharides, and their maturation occurs in the Golgi apparatus by trimming with endogenous glycosidases. They are then modified by addition of complex oligosaccharides and terminal sialic acid through glycosyltransferases.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Placenta
/
Gonadotropina Coriónica
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
J Biochem
Año:
1987
Tipo del documento:
Article